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>Isolasi, Skrining Dan Identifikasi Jamur Xilanolitik Lokal Yang Berpotensi Sebagai Agensia Pemutih Pulp Yang Ramah Lingkungan (Isolation, Screening and Identification Xylanolytic Local Fungi That Potentially as Pulp Bleaching Agents)
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Isolasi, Skrining Dan Identifikasi Jamur Xilanolitik Lokal Yang Berpotensi Sebagai Agensia Pemutih Pulp Yang Ramah Lingkungan (Isolation, Screening and Identification Xylanolytic Local Fungi That Potentially as Pulp Bleaching Agents)
Xylanase has great potential for industry application. Application of xylanase can be done in pretreatment of pulp bleaching in the pulp and paper industry. Enzyme application can reduce the use of chlorine compounds that are harmful to the environment. Therefore, xylanase that used in pulp bleaching should be free of cellulase activity. Fungi are one of the groups of microbes that are able to produce xylanase. The aims of this study was to obtain local xylanase-producing fungal isolates from soil that assumed contain of xylan. The source of fungal isolates were the soil around the pulp and paper industry; Acacia forests in the district Ogan Ilir and Muara Enim, South Sumatra; Wanagama, Yogyakarta; sawmills in Palembang and Yogyakarta; and Palembang landfill. Based on the initial screening in the agar basal medium, 111 fungal isolates were obtained. Most of them were the xylanase-producing fungi, but only 12 fungal isolates that have high xylanolytic capabilities. Further screening was performed on xylan liquid basal medium. The results showed that the fungus identified as Chaetomium globosum, Penicillium simplicissimum, Aspergillus tamarii and Monocillium have higher xylanase specific activity than the other isolates. They were also have lignolytic and cellulolytic activities. Therefore, fungal xylanase potentially developed as a pulp bleaching agent.
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